Environmental DNA in water

Frode Fossøy demonstrates how to take eDNA-samples. English subtitles in YouTube-settings.

Environmental DNA in water

The vast majority of people who have heard of environmental DNA (e-DNA) usually associate this with water samples. By filtering water through a fine-mesh filter, we can collect DNA residues floating around in the water, and with genetic analyses we can determine which species it comes from.

NINA offers our own e-DNA kit for taking water samples in rivers and lakes. With this simple kit, anyone can collect e-DNA samples they submit for analysis at NINA. If you want to use our e-DNA analyses, please contact Frode Fossøy or Rolf Sivertsgård.

We use species-specific markers to detect individual species and general-species markers to describe the species diversity of species groups in the aquatic environment. Species-specific analyses use a genetic marker that is only found in the species we are looking for using a qPCR or digital-PCR machine. General-species analyses use a genetic marker found in many species, and we often want to investigate a specific group such as fish, amphibians, insects or mammals. We then use a DNA metabarcoding analysis (DNA metabarcoding) where we read millions of short DNA fragments that we then compare with a reference database to determine which species each DNA fragment originates from.



Andersson AF, Bissett A, Finstad AG, Fossøy F, Grosjean M, Hope M, Jeppesen TS, Kõljalg U, Lundin D, Nilsson RN, Prager M, Svenningsen C & Schigel D (2021) Publishing DNA-derived data through biodiversity data platforms. v1.0 Copenhagen: GBIF Secretariat.

Gargan L, Fossøy, F, Mo, T.A., Carlsson J.E.L, Ball B & Carlsson J. 2021. Development of an environmental DNA assay and field validation for the detection of invasive pink salmon Oncorhynchus gorbuscha. Environmental DNA doi.org/10.1002/edn3.250.

Taugbøl, A, Bærum, KM, Dervo, BK & Fossøy, F. 2021. The first detection of the fungal pathogen batrachochytrium dendrobatidis in Norway with no evidence of population declines for great crested and smooth newts based on modeling on traditional trapping data.Environmental DNA 10.1002/edn3.180.